KMID : 0613820070170101347
|
|
Journal of Life Science 2007 Volume.17 No. 10 p.1347 ~ p.1353
|
|
Changes of Glycosylation Pattern in Aging Rat Kidneys as Revealed with Lectin Conjugates
|
|
Gil Young-Gi
Kim Keun-Ha Choi Byung-Tae
|
|
Abstract
|
|
|
The changes of glycoconjuagates (GCs) in rat kidney due to maturation were studied from samples of fetal and postnatal kidneys by lectin histochemistry. Rat kidneys of perinatal ages and adults were fixed in 4% paraformaldehyde and were stained with nine kinds of biotinylated lectins. The immature forms of the renal developmental stage such as vesicles and ureteric bud were observed in the cortex as late as day 14 of postnatal life, but the histological appearance of the weaning kidney was similar to that observed in adults. As for histochemical properties of GCs in the glomeruli, Con A affinity tended to increase with aging, but both RCA-1 and LCA affinities showed a transient increase in immature glomeruli of neonatal rats. DBA affinity with SBA, PNA, BSL-1 and RCA-1, additional Con A one in proximal tubule, were increased in both proximal and distal tubules according to maturation. In contrast to this, transient intensive LCA affinity were demonstrated in immature proximal and distal tubule of neonatal rats. In the collecting tubules, DBA, SBA, PNA and sWGA affinities tended to increase according to maturation, but transient increase for BSL-1, RCA-1 and LCA affinities were detected in neonatal rats. The present results suggest that the mature glycosylation pattern of the kidney undergoes profound changes during maturation and is probably associated with functional maturation of the kidney.
|
|
KEYWORD
|
|
Glycoconjuagtes, lectin, kidney, development, rat
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|